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Figure 2. Effect of BoNT/A on astroglial calcium-binding protein <t>S100β</t> (S100β) expression: (A) Image depicting immunofluorescence staining (scale bar = 150 µm; 200× magnification). (B) ELISA results revealed significantly higher S100β levels in the IT and DT groups compared with that in the control, with a significant difference being observed between the IT and DT groups at week 6. * indicates IT group vs. control group, ∆indicates IT group vs. DT group, and # indicates DT group vs. control group (p < 0.05). n = 5 in each group. The error bars indicate the standard error of the mean.
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Figure 2. Effect of BoNT/A on astroglial calcium-binding protein <t>S100β</t> (S100β) expression: (A) Image depicting immunofluorescence staining (scale bar = 150 µm; 200× magnification). (B) ELISA results revealed significantly higher S100β levels in the IT and DT groups compared with that in the control, with a significant difference being observed between the IT and DT groups at week 6. * indicates IT group vs. control group, ∆indicates IT group vs. DT group, and # indicates DT group vs. control group (p < 0.05). n = 5 in each group. The error bars indicate the standard error of the mean.
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Figure 2. Effect of BoNT/A on astroglial calcium-binding protein <t>S100β</t> (S100β) expression: (A) Image depicting immunofluorescence staining (scale bar = 150 µm; 200× magnification). (B) ELISA results revealed significantly higher S100β levels in the IT and DT groups compared with that in the control, with a significant difference being observed between the IT and DT groups at week 6. * indicates IT group vs. control group, ∆indicates IT group vs. DT group, and # indicates DT group vs. control group (p < 0.05). n = 5 in each group. The error bars indicate the standard error of the mean.
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Figure 2. Effect of BoNT/A on astroglial calcium-binding protein <t>S100β</t> (S100β) expression: (A) Image depicting immunofluorescence staining (scale bar = 150 µm; 200× magnification). (B) ELISA results revealed significantly higher S100β levels in the IT and DT groups compared with that in the control, with a significant difference being observed between the IT and DT groups at week 6. * indicates IT group vs. control group, ∆indicates IT group vs. DT group, and # indicates DT group vs. control group (p < 0.05). n = 5 in each group. The error bars indicate the standard error of the mean.
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Figure 2. Effect of BoNT/A on astroglial calcium-binding protein <t>S100β</t> (S100β) expression: (A) Image depicting immunofluorescence staining (scale bar = 150 µm; 200× magnification). (B) ELISA results revealed significantly higher S100β levels in the IT and DT groups compared with that in the control, with a significant difference being observed between the IT and DT groups at week 6. * indicates IT group vs. control group, ∆indicates IT group vs. DT group, and # indicates DT group vs. control group (p < 0.05). n = 5 in each group. The error bars indicate the standard error of the mean.
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Figure 2. Effect of BoNT/A on astroglial calcium-binding protein S100β (S100β) expression: (A) Image depicting immunofluorescence staining (scale bar = 150 µm; 200× magnification). (B) ELISA results revealed significantly higher S100β levels in the IT and DT groups compared with that in the control, with a significant difference being observed between the IT and DT groups at week 6. * indicates IT group vs. control group, ∆indicates IT group vs. DT group, and # indicates DT group vs. control group (p < 0.05). n = 5 in each group. The error bars indicate the standard error of the mean.

Journal: Toxins

Article Title: Comparison of Neural Recovery Effects of Botulinum Toxin Based on Administration Timing in Sciatic Nerve-Injured Rats.

doi: 10.3390/toxins16090387

Figure Lengend Snippet: Figure 2. Effect of BoNT/A on astroglial calcium-binding protein S100β (S100β) expression: (A) Image depicting immunofluorescence staining (scale bar = 150 µm; 200× magnification). (B) ELISA results revealed significantly higher S100β levels in the IT and DT groups compared with that in the control, with a significant difference being observed between the IT and DT groups at week 6. * indicates IT group vs. control group, ∆indicates IT group vs. DT group, and # indicates DT group vs. control group (p < 0.05). n = 5 in each group. The error bars indicate the standard error of the mean.

Article Snippet: ELISA kits for GFAP [CUSABIO, CSB-E08602r], S100β [CUSABIO, CSB-E08066r], GAP43 [CUSABIO, CSB-EL009231RA], NF200 [MyBioSource, MBS3808264], and BDNF [CUSABIO, CSB-E04685r] were used in accordance with the manufacturer’s instructions to measure the levels of these proteins in the frozen sciatic nerve tissue.

Techniques: Binding Assay, Expressing, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Control